gotaq® flexi buffer (Promega)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Promega gotaq® flexi buffer
Gotaq® Flexi Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gotaq+flexi+buffer/gotaq+qpcr+master+mix/pm40276324-91-13-17
Average 90 stars, based on 1 article reviews

gotaq® flexi buffer - by Bioz Stars, 2026-10

90/100 stars

Images

Polymerase Chain Reaction:

Article Title: A First Approximation of Genetic Diversity and Population Structure to Identify Management Units for Western North Carolina Timber Rattlesnakes (Crotalus horridus)
Article Snippet: .. PCR reactions were prepared in 10μL volumes consisting of GoTaq Flexi Buffer, 2.5 mM MgCl2, 800 μM dNTPs, 0.5 μM of reverse primer, 0.25 μM of tagged forward primer, 0.25 μM of a M13 uorescent labeled primer, 0.5 units of GoTaq Flexi DNA Polymerase, and ~ 30ng of DNA (Promega, Madison, Wisconsin, USA). .. A touchdown PCR protocol was employed to increase speci city and reduce stutter on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hauppauge, NY, USA) (Korbie and Mattick, 2008).

Article Title: Novel protocol for mapping virus integration sites in genes involved in therapy resistance.
Article Snippet: .. The PCR was performed on a Biorad T100 thermal cycler using a 50 μL PCR reaction mix containing 1X GoTaq Flexi Buffer (Promega, cat.no M890A), 2 mM MgCl2 (Promega, cat.no A351H), 0.2 mM dNTPs (NEB, cat. No N0447S), 1.0 μM neomycinspecific primer (NeoP), 1.0 μM locus-specific primer (LSP), 1.25 U GoTaq G2 Flexi DNA polymerase (Promega, cat.no M780B), 10% Glycerol (Sigma-Aldrich, cat. No. G5516), UltraPureTM Distilled Water (Invitrogen, cat. No 10977035) and 10 ng gDNA. ..

Labeling:

Nucleic Acid Electrophoresis:

Article Title: Evolutionary and dispersal dynamics in invasive races of Puccinia striiformis f. sp. tritici attributed to virulence and SCAR markers
Article Snippet: Highly virulent races of Puccinia striiformis f. sp. tritici (Pst) are driving the spread of yellow rust epidemics across continents.. From 2021 to 2023, this study analyzed 94 single-pustule isolates of Pst collected from wheat samples across four provinces in Egypt, identifying 40 distinct physiological races, including eight reported for the first time (12E138, 174E191, 206E191, 6E143, 6E153, 78E156, 78E159, and 78E191).. Five genetic lineages (PstS2, PstS3, PstS12, PstS14, and PstS17) were characterized, with the new aggressive race PstS17 reported for the first time, alongside new virulence formulas for races PstS3v3v4, PstS12v4, PstS14v4, and PstS17v4.

Amplification:

Article Title: First record of Isospora amphiboluri in the thorny devil, Moloch horridus
Article Snippet: .. A region (∼810 bp) of cox 1 was amplified employing primers KM204 (forward: 5′- GTT TGG TTC AGG TGT TGG TTG -′3) and KM205 (reverse: 5′- ATC CAA TAA CCG CAC CAA GAG -′3) ( ) using the following cycling protocol: 94 °C for 5 min (initial denaturation), followed by 35 cycles of 94 °C for 30 s (denaturation), 55 °C for 45 s (annealing) and 72 °C for 45 s (extension), with a final extension of 72 °C for 5 min. A partial region (∼1500 bp) of SSU was amplified employing primers EIF1 (forward: 5′- GCT TGT CTC AAA GAT TAA GCC -′3) and EIF3 (reverse: 5′- ATG CAT ACT CAA AAG ATT ACC -3′) ( ) using the following cycling protocol: 94 °C for 5 min (initial denaturation), followed by 35 cycles of 94 °C for 30 s (denaturation), 60 °C for 45 s (annealing) and 72 °C for 90 s (extension), with a final extension of 72 °C for 7 min. All PCRs were conducted in a volume of 50 μl including 2 μl of genomic DNA, the GoTaq Flexi buffer (Promega Australia, Alexandria, New South Wales, Australia), 3.0 mM of MgCl 2 , 200 μM of each deoxynucleotide triphosphate (dNTP), 25 pmol of each primer and 1 U of GoTaq DNA polymerase (Promega). ..

other:

Article Title: A High Frequency of Detection of Recombinant Koala Retrovirus (recKoRV) in Victorian Koalas Suggests Historic Integration of KoRV
Article Snippet: Each reaction mixture consisted of 5 μL template, 500 nM of each primer, 0.20 mM of each dNTP, 2.00 mM MgCl 2 , 1.5 units GoTaq Flexi DNA Polymerase (Promega), and 1 × GoTaq Flexi buffer.

Purification:

Article Title: First Confirmed Occurrence of Ciguatera Poisoning in the UK from Imported Pinjalo Snapper ( Pinjalo pinjalo )
Article Snippet: .. Amplifications were performed in a 50 μL reaction volume consisting of 1× GoTaq flexi buffer (Promega, Southampton, UK), 2.5 mM MgCl 2 (Merck, Poole, UK), 1 mM dNTP mix, 50 pmol of the forward and reverse primers, 1.25 units of GoTaq DNA Polymerase (Promega, Southampton, UK) and 2.5 μL of the purified DNA template (SOP 2007). .. The reaction mix was overlaid with mineral oil and after an initial denaturing step (5 min at 95 °C), was subjected to 40 temperature cycles (1 min at 95 °C, 1 min at 60 °C and 1 min at 72 °C) in a Peltier PTC-225 thermal cycler (MJ Research, Braintree, UK) followed by a final extension step of 10 min at 72 °C.

Review